Molecular Signaling Pathways: How Genes Are Linked To Behavior

Prof. Richard C. Deth, Department of Pharmaceutical Sciences


Lecture Overview:

Within biological systems, the concentration of signaling molecules represents a source of information for the regulation of biochemical processes and electrical events. Important categories of signaling molecules include hormones, neurotransmitters and growth factors, each interacting with specific receptor proteins that translate their concentration into cellular effects. This lecture will review the molecular basis for signaling via several of the major receptor-initiated pathways, with an emphasis on the structural features that provide for transmission of signals. Signaling by the D4 dopamine receptor provides an interesting example of how genetic diversity can influence a complex behavior (i.e. the capacity for attention). Polymorphisms in the D4 receptor gene found only in primates are associated with novelty-seeking behavior and also with the risk of attention-deficit hyperactivity disorder (ADHD). An understanding of the unique features of this receptor, and their role in signaling, allows a molecular hypothesis for attention with implications for the origins of several neuropsychiatric disorders.

Laboratory Overview

Molecular modeling, including 3-D molecular graphics, provides a powerful technology for understanding molecular architecture and assisting in the design of new drugs. In this laboratory activity, students will be introduced to the molecular modeling environment and will learn to use basic tools to examine the structural features of signaling molecules, including neurotransmitters, hormones and their respective receptors and other relevant signaling intermediates. The importance of molecular conformation and energetic considerations in ligand-receptor interactions will also be introduced.

Student Assigment

Students will determine the lowest energy conformation of several neurotransmitters and prepare a brief report comparing these conformations.